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Sekisui XenoTech
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Covalab Inc
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MBL Life science
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MacVector inc
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Becton Dickinson
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KangChen Inc
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Image Search Results
Journal: Journal of Innate Immunity
Article Title: Expression of MIG/CXCL9 in Cystic Fibrosis and Modulation of Its Activities by Elastase of Pseudomonas aeruginosa
doi: 10.1159/000365399
Figure Lengend Snippet: The distribution of MIG/CXCL9 in CF lung tissue as detected by immunoelectron microscopy. Ultrathin sections from CF lung specimens were incubated with specific antibodies against MIG/CXCL9. Bound antibodies were visualized using secondary goat anti-rabbit antibodies conjugated with colloidal gold particles (10 nm). a In large airways, ciliated epithelium and goblet cells outline the lumen that shows presence of necrotic and disrupted neutrophils and mucus. b MIG/CXCL9 is detected on the surface of bronchial epithelial cells and on cilia as reflected by immunogold particles. c In small airways (i.e. bronchioli), a similar distribution of MIG/CXCL9 is observed with the presence on the surface of epithelial cells as well as in vesicles present in the cytoplasm (arrow). The latter may represent pinocytosis or export of de novo synthesized MIG/CXCL9. d Only low levels of MIG/CXCL9 were detected in alveoli as visualized by scant colloidal gold particles. e Presence of MIG/CXCL9 was also seen in airway mucus. f, g Replacement of the primary antibodies with preimmune IgG resulted in loss of labeling. The bars represent 1 μm (a), 250 nm (b, d, f, g), and 100 nm (c, e).
Article Snippet: Recombinant human MIG/CXCL9, affinity-purified polyclonal antibodies against human MIG/CXCL9 and
Techniques: Immuno-Electron Microscopy, Incubation, Synthesized, Labeling